Acute effect of gamma irradiation in mice by NMR based metabolic profiling of urine

Introduction: Radiation casualty management relies on clinical signs and symptoms, biological assessments, radiation exposure and physical dosimetry. No single assay is sufficient to address all potential radiation scenarios including mass casualties. A quick, non/minimally invasive, multiparameter bioassay strategy based on early response from radiation exposure is needed to adequately assess doses in accidental and occupational overexposure cases. Earlier we have detected NMR spectroscopy based some potential and promising response at metabolite levels in serum samples from mice when exposed to radiation stress. However, for development of non invasive techniques for identification of metabolic markers for radiation related changes in large number of samples, NMR spectroscopy based metabolic profiles of urine is far feasible. Urine spectrum provides a great deal of information by expressing a range of metabolites from energy metabolites to osmolytes and nucleotide metabolites to amino acids. The present study has been proposed to exhibit the time dependent perturbation of metabolism in mice urine due to acute gamma radiation injury.